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Generation of Single-Cell Suspensions from Mouse Neural Tissue
Chapters
- 00:05Title
- 01:08Introduction
- 01:43Preparation of Reagents
- 04:00The gentleMACS Tissue Dissociation
- 07:22Filtration
- 09:35Representative Results
- 10:25Conclusion
Dissociating cells from specific tissue types requires specific parameters for tissue aggitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS Dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on nerual tissue is explained.
Tags
Single-cell SuspensionsNervous SystemNeuronal Cell TypesGlial Cell TypesCell Surface MoleculesMolecular DeterminantsCell IdentificationCell Separation TechnologiesGentleMACS DissociatorTrypsin-based Dissociation KitsPapain-based Dissociation KitsAntigen EpitopesCell LossC TubesGentleMACS ProgramsMonoclonal ConjugatesAnti-Prominin-1 MicroBeadsNeural ProgenitorsMyelin Removal Beads
