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Generation of Single-Cell Suspensions from Mouse Neural Tissue
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July 07, 2009
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Dissociating cells from specific tissue types requires specific parameters for tissue aggitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS Dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on nerual tissue is explained.
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Pennartz, S., Reiss, S., Biloune, R., Hasselmann, D., Bosio, A. Generation of Single-Cell Suspensions from Mouse Neural Tissue. J. Vis. Exp. (29), e1267, doi:10.3791/1267 (2009).
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