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In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum

In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum

DOI:
10.3791/1210-v

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08:53 min

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April 29, 2009

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, ,
1Biology Research and Development ,Caliper Life Sciences

Chapters

  • 00:00Title
  • 00:48Introduction
  • 01:15Cell Preparation
  • 02:57Animal Injection
  • 04:06Animal Imaging
  • 06:27Time Course
  • 07:51Conclusion

Summary

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Mammary tumor cells expressing luciferase are implanted subcutaneously in mice and visualized using optical imaging to monitor tumor growth and development non-invasively in a longitudinal study.

Tags

In Vivo Bioluminescent ImagingMammary TumorsIVIS Spectrum4T1 Mouse Mammary Tumor CellsXenograft Tumor ModelPre-clinical EvaluationAntitumor CompoundsFirefly Luciferase GeneLuciferin InjectionOptical Imaging SystemPhoton FluxAbsolute QuantitationLongitudinal StudiesTumor Growth MonitoringEarly Events In Tumor DevelopmentBioluminescent Signal ReductionTumor Cell DeathHypoxiaDrug Treatment

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