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The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

DOI:
10.3791/1026-v

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08:15 min

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January 06, 2009

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, ,
1Gene Expression Division,Bio-Rad Laboratories, Inc

Chapters

  • 00:00Title
  • 00:08Introduction
  • 00:43Harvesting Bone Marrow From Femurs and Tibiae
  • 02:07Establishing Cultures
  • 04:33Electroporating Cells
  • 07:13Transfection Results
  • 07:53Conclusion

Summary

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This procedure describes how to establish primary hematopoietic cell cultures from murine bone marrow and is followed by transfection using the Gene Pulser MXCell electroporation system.

Tags

Primary Hematopoietic Cell CulturesMurine Bone MarrowElectroporationPlasmid DNASiRNAGene Pulser MXcell Electroporation SystemGene Pulser Electroporation BufferTransfectionNucleic AcidsMammalian CellsDifficult-to-transfect CellsStem CellsVideo DemonstrationFemurTibia

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