Assessment of Cornea and Tear Smoothness in an Autoimmune Dry Eye Rat Model

Published: October 31, 2023

Abstract

Source: Hou, A. et al., A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue. J. Vis. Exp. (2017)

This video demonstrates a method for assessing corneal smoothness and tear film stability in an autoimmune rat model. It highlights the impact of gland dysfunction on tear film stability, crucial for understanding dry eye disease.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Lacrimal Gland Extract NOTE: Rats were anesthetized with the intraperitoneal injection of ketamine (75 mg/kg) and xylazine (10 mg/kg). Proper anesthetization was confirmed by toe pinching and tail pinching. Ophthalmic gel was applied to the rat's eyes to prevent dryness …

Disclosures

The authors have nothing to disclose.

Materials

Reagents
Protease inhibitor cocktail Sigma-Aldrich P2714-1BTL
Pierce BCA Protein Assay Kit Thermal Scientific 23227
Mycobacterium tuberculosis H37Ra  Becton, Dickinson and company 231141
Complete Freund's adjuvant Becton, Dickinson and company 231131
Ovalbumin Sigma-Aldrich A5503-10G
Incomplete Freund's adjuvant  Sigma-Aldrich F5506-6X10ML
Pertussis toxin  Sigma-Aldrich P7208-50UG
Equipment
Sonicator Sonics  Vibra-Cell
Stereo microscope with ring light illuminator and camera Carl Zeiss NA

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Cite This Article
Assessment of Cornea and Tear Smoothness in an Autoimmune Dry Eye Rat Model. J. Vis. Exp. (Pending Publication), e21765, doi: (2023).

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