Assessment of Cornea and Tear Smoothness in an Autoimmune Dry Eye Rat Model
Published: October 31, 2023
Abstract
Source: Hou, A. et al., A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue. J. Vis. Exp. (2017)
This video demonstrates a method for assessing corneal smoothness and tear film stability in an autoimmune rat model. It highlights the impact of gland dysfunction on tear film stability, crucial for understanding dry eye disease.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Lacrimal Gland Extract NOTE: Rats were anesthetized with the intraperitoneal injection of ketamine (75 mg/kg) and xylazine (10 mg/kg). Proper anesthetization was confirmed by toe pinching and tail pinching. Ophthalmic gel was applied to the rat's eyes to prevent dryness …
Disclosures
The authors have nothing to disclose.
Materials
| Reagents | |||
| Protease inhibitor cocktail | Sigma-Aldrich | P2714-1BTL | |
| Pierce BCA Protein Assay Kit | Thermal Scientific | 23227 | |
| Mycobacterium tuberculosis H37Ra | Becton, Dickinson and company | 231141 | |
| Complete Freund's adjuvant | Becton, Dickinson and company | 231131 | |
| Ovalbumin | Sigma-Aldrich | A5503-10G | |
| Incomplete Freund's adjuvant | Sigma-Aldrich | F5506-6X10ML | |
| Pertussis toxin | Sigma-Aldrich | P7208-50UG | |
| Equipment | |||
| Sonicator | Sonics | Vibra-Cell | |
| Stereo microscope with ring light illuminator and camera | Carl Zeiss | NA |
Tags
Cite This Article
Assessment of Cornea and Tear Smoothness in an Autoimmune Dry Eye Rat Model. J. Vis. Exp. (Pending Publication), e21765, doi: (2023).