JOVE JOURNAL

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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Cheryl A. Hobbs*¹, Leslie Recio¹, John Winters¹, Kristine L. Witt²

¹Toxicology Program, ILS, Inc., ²Division of the National Toxicology Program,National Institute of Environmental Health Sciences

Journal (Genetics)

A Screening Method for Identification of Heterochromatin-Promoting Drugs Using Drosophila

Lin Zhang¹, Kenny Dao¹, Angela Kang¹, Andre C. Loyola¹, Robin Shang¹, Jinghong Li¹, Willis X. Li¹

¹Department of Medicine,University of California San Diego

Journal (Genetics)

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Bas B. Oude Munnink¹, David F. Nieuwenhuijse¹, Reina S. Sikkema¹, Marion Koopmans¹

¹ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research,Erasmus University Medical Center

Journal (Genetics)

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Yaolei Mi^1,2, Zhihui Zhu³, Guangtao Qian³, Yu Li⁴, Xiangxiao Meng¹, Jianping Xue³, Qingfu Chen⁵, Wei Sun¹, Yuhua Shi^1,2

¹Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences, ²Artemisinin Research Center,China Academy of Chinese Medical Sciences, ³College of Life Science,Huaibei Normal University, ⁴Economic Crop Research Institute Sichuan Academy of Agriculture Sciences, ⁵Research Center of Buckwheat Industry Technology,Guizhou Normal University

Journal (Genetics)

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Rafael J. L. Morcillo¹, Achen Zhao^1,2, María I. Tamayo-Navarrete³, José M. García-Garrido³, Alberto P. Macho¹

¹Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences, ²University of Chinese Academy of Sciences, ³Department of Soil Microbiology and Symbiotic Systems,Estación Experimental del Zaidín (CSIC)

Journal (Genetics)

Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence

Sean J. O'Sullivan^1,2, Beverly A.S. Reyes³, Rajanikanth Vadigepalli¹, Elisabeth J. Van Bockstaele³, James S. Schwaber¹

¹Daniel Baugh Institute for Functional Genomics and Computational Biology, Department of Pathology, Anatomy, and Cell Biology,Thomas Jefferson University, ²Sidney Kimmel Medical College,Thomas Jefferson University, ³Department of Pharmacology & Physiology,Drexel University College of Medicine

Journal (Genetics)

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Francis Migneault^1,2,3, Frédéric Gagnon^2,3, Emmanuelle Brochiero^1,3, Yves Berthiaume^2,3, André Dagenais^2,3

¹Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), ²Institut de Recherches Cliniques de Montréal (IRCM), ³Département de médecine, Faculté de médecine,Université de Montréal

Journal (Genetics)

Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

Yana P. Blokhina^1,2, Ivan Olaya^1,2, Sean M. Burgess¹

¹Department of Molecular and Cellular Biology,University of California, Davis, ²Integrative Genetics and Genomics Graduate Group,University of California, Davis

Journal (Genetics)

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

Anne-Marlen Gaebler¹, Alexander Hennig¹, Katharina Buczolich¹, Jürgen Weitz¹, Thilo Welsch¹, Daniel E. Stange¹, Kristin Pape¹

¹Department of Visceral-, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus,Technische Universität Dresden

Journal (Genetics)

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

Nandaraj Taye¹, Sarah Stanley¹, Dirk Hubmacher¹

¹Orthopaedic Research Laboratories, Leni & Peter W. May Department of Orthopaedics,Icahn School of Medicine at Mount Sinai

Journal (Genetics)

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Jinxia Shi¹, Yijuan Jia¹, Di Fang¹, Shidan He¹, Peng Zhang^1,2, Yushuang Guo³, Yongli Qiao¹

¹Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences,Shanghai Normal University, ²College of Agriculture,Yangtze University, ³Laboratory of Molecular Genetics, China National Tobacco Corporation,Guizhou Institute of Tobacco Science

Journal (Genetics)

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Snehadri Sinha*^1,2, Bettina Mannerström*^1,2, Riitta Seppänen-Kaijansinkko^1,2, Sippy Kaur^1,2

¹Department of Oral and Maxillofacial Diseases,University of Helsinki, ²Helsinki University Hospital

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