JOVE JOURNAL

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Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Yelena Levin*¹, Keyur Talsania*^1,2, Bao Tran¹, Jyoti Shetty¹, Yongmei Zhao^1,2, Monika Mehta¹

¹NCI CCR Sequencing Facility,Frederick National Laboratory for Cancer Research, ²Advanced Biomedical and Computational Sciences,Frederick National Laboratory for Cancer Research

Journal (Genetics)

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Sumana Sharma^1,2, Gavin J. Wright¹

¹Cell Surface Signalling Laboratory,Wellcome Trust Sanger Institute, ²EMBL-EBI,Wellcome Genome Campus

Journal (Genetics)

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

Laetitia Chauve*¹, Jérémie Le Pen*^2,3,5, Francesca Hodge¹, Pia Todtenhaupt¹, Laura Biggins¹, Eric A. Miska^2,3,4, Simon Andrews¹, Olivia Casanueva¹

¹Babraham Institute, ²Gurdon Institute,University of Cambridge, ³Department of Genetics,University of Cambridge, ⁴Wellcome Trust Genome Campus,Wellcome Trust Sanger Institute, ⁵Laboratory of Virology and Infectious Disease,The Rockefeller University

Journal (Genetics)

Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus

Michelle Bui¹, Ming Li¹, Robyn R Raban¹, Nannan Liu², Omar S Akbari^1,3

¹Section of Cell and Developmental Biology,University of California, San Diego, ²Department of Entomology and Plant Patholog,Auburn University, ³Tata Institute for Genetics and Society,University of California, San Diego

Journal (Genetics)

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification

Xianju Bi^1,2, Xuechun Zhang¹, Xiaohua Shen¹

¹Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences,Tsinghua University, ²Department of Molecular Biology,University of Texas Southwestern Medical Center

Journal (Genetics)

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Jos G.A. Smits*¹, Jieqiong Qu*¹, Hanna Niehues², Huiqing Zhou^1,3

¹Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences,Radboud University, Nijmegen, The Netherlands, ²Department of Dermatology, Radboud University Medical Center,Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands, ³Department of Human Genetics,Radboud University Medical Center, Nijmegen, The Netherlands

Journal (Genetics)

Methylated RNA Immunoprecipitation Assay to Study m⁵C Modification in Arabidopsis

Eleftheria Saplaoura*¹, Valentina Perrera*², Vincent Colot³, Friedrich Kragler¹

¹Max Planck Institute of Molecular Plant Physiology, ²Department of Molecular Medicine, Medical School,University of Padua, ³Biologie de l'Ecole Normale Supérieure (IBENS), Paris, France

Journal (Genetics)

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Yu Wang¹, Andrew J. H. Smith¹, David C. Hay¹

¹MRC Centre for Regenerative Medicine,University of Edinburgh

Journal (Genetics)

Exon Skipping in Directly Reprogrammed Myotubes Obtained from Human Urine-Derived Cells

Hotake Takizawa¹, Mitsuto Sato¹, Yoshitsugu Aoki¹

¹Department of Molecular Therapy, National Institute of Neuroscience,National Center of Neurology and Psychiatry

Journal (Genetics)

Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)

Tian V. Tian^1,2,3, Enrique Vidal^1,2, Thomas Graf^1,2, Grégoire Stik^1,2

¹Center for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology, ²Universitat Pompeu Fabra, ³Vall d'Hebron Institute of Oncology

Journal (Genetics)

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Hirofumi Nishizono*^1,2,3, Mohamed Darwish*^4,5, Hideki Uosaki^6,7, Nanami Masuyama^8,9,10, Motoaki Seki^8,11, Hiroyuki Abe³, Nozomu Yachie^8,9,10,12,13, Ryohei Yasuda¹

¹Max Planck Florida Institute for Neuroscience, ²Life Science Research Center,University of Toyama, ³Department of Biochemical Engineering, Graduate School of Science and Engineering,Yamagata University, ⁴Graduate School of Innovative Life Science,University of Toyama, ⁵Department of Biochemistry, Faculty of Pharmacy,Cairo University, ⁶Division of Regenerative Medicine, Center for Molecular Medicine,Jichi Medical University, ⁷Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology,Jichi Medical University, ⁸Synthetic Biology Division, Research Center for Advanced Science and Technology,University of Tokyo, ⁹Institute for Advanced Biosciences,Keio University, ¹⁰Graduate School of Media and Governance,Keio University, ¹¹Department of Molecular Oncology, Graduate School of Medicine,Chiba University, ¹²Department of Biological Sciences, School of Science,University of Tokyo, ¹³College of Arts and Sciences,University of Tokyo

Journal (Genetics)

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Krzysztof J. Skowronek¹, Matthias Bochtler¹

¹International Institute of Molecular and Cell Biology, Warsaw (IIMCB)

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