JOVE JOURNAL

生物化学

Showing 493 - 504 in 778 videos.

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

Patrick Moran^1,2, Yichen Guo^3,4, Rong Yuan^1,3, Nicholas Barnekow¹, Jordan Palmer², Adam Beck³, Bin Ren^3,4,5

¹Blood Research Institute,Blood Center of Wisconsin, ²Department of Medicine,Medical College of Wisconsin, ³Department of Surgery,The University of Alabama at Birmingham, ⁴Department of Biomedical Engineering,The University of Alabama at Birmingham, ⁵GBS Program, Graduate School,The University of Alabama at Birmingham

Journal (Biochemistry)

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes

Alexanne Cuillerier¹, Yan Burelle¹

¹Interdisciplinary School of Health Science, Faculty of Health Sciences and Department of Cellular and Molecular Medicine, Faculty of Medicine,University of Ottawa

Journal (Biochemistry)

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

Shellaina J.V. Gordon*¹, Yao Xiao*², Amanda L. Paskavitz³, Napoleón Navarro-Tito⁴, Juan G. Navea², Teresita Padilla-Benavides¹

¹Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, ²Department of Chemistry,Skidmore College, ³Candiac MR Center,Beth Israel Deaconess Medical Center, ⁴Facultad de Ciencias Químico Biológicas,Universidad Autónoma de Guerrero

Journal (Biochemistry)

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Phillip C. Delekta¹, Todd A. Lydic², Neal D. Hammer¹

¹Department of Microbiology and Molecular Genetics,Michigan State University, ²Department of Physiology,Michigan State University

Journal (Biochemistry)

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Chase M. Clark¹, Maria S. Costa^1,2, Erin Conley¹, Emma Li¹, Laura M. Sanchez¹, Brian T. Murphy¹

¹Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy,University of Illinois at Chicago, ²Faculty of Pharmaceutical Sciences,University of Iceland

Journal (Biochemistry)

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Markus Axmann¹, Andreas Karner², Sabine M. Meier¹, Herbert Stangl¹, Birgit Plochberger²

¹Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry,Medical University Vienna, ²School of Medical Engineering and Applied Social Sciences,University of Applied Sciences Upper Austria

Journal (Biochemistry)

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

Ingo Bauer¹, Angelo Pidroni¹, Özgür Bayram^2,3, Gerald Brosch¹, Stefan Graessle¹

¹Division of Molecular Biology, Biocenter,Medical University of Innsbruck, ²Biology Department,Maynooth University, ³Maynooth University Human Health Research Institute

Journal (Biochemistry)

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Rui Yin*, Catherine Harvey*, Diane C. Shakes*, Oliver Kerscher*

¹Integrated Science Center, Biology Department,College of William & Mary

Journal (Biochemistry)

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Alex Y. H. Wong¹, Sheng Xie¹, Ben Zhong Tang², Sijie Chen¹

¹Ming Wai Lau Centre for Reparative Medicine,Karolinska Institutet, ²Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science,Hong Kong University of Science and Technology

Journal (Biochemistry)

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Simon Leclerc^1,2, Youri Arntz², Yuichi Taniguchi^1,3

¹Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research,RIKEN, ²Laboratoire de Biomatériaux et Bioimagerie,INSERM, ³PRESTO, Japan Science and Technology Agency

Journal (Biochemistry)

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Fatima Enam¹, Thomas J. Mansell¹

¹Department of Chemical and Biological Engineering,Iowa State University

Journal (Biochemistry)

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Yu-Ting Huang^1,2, Dinja van der Hoorn*^1,2, Leire M. Ledahawsky*^1,2, Anna A. L. Motyl*^1,2, Crispin Y. Jordan¹, Thomas H. Gillingwater^1,2, Ewout J. N. Groen^1,2

¹Centre for Discovery Brain Sciences,University of Edinburgh, ²Euan MacDonald Centre for Motor Neurone Disease Research,University of Edinburgh

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