JOVE JOURNAL

生物化学

Showing 517 - 528 in 778 videos.

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Journal (Biochemistry)

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Kentaro Ito¹, Bilge Argunhan¹, Hideo Tsubouchi¹, Hiroshi Iwasaki¹

¹Cell Biology Center, Institute of Innovative Research,Tokyo Institute of Technology

Journal (Biochemistry)

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Alex Kirui*¹, Malitha C. Dickwella Widanage*¹, Frederic Mentink-Vigier², Ping Wang³, Xue Kang¹, Tuo Wang¹

¹Department of Chemistry,Louisiana State University, ²National High Magnetic Field Laboratory, ³Departments of Pediatrics, and Microbiology, Immunology and Parasitology,Louisiana State University Health Sciences Center

Journal (Biochemistry)

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Nicholas McCaul^1,2, Hui Ying Yeoh¹, Guus van Zadelhoff¹, Naomi Lodder¹, Bertrand Kleizen¹, Ineke Braakman¹

¹Cellular Protein Chemistry,Utrecht University, ²Program in Cellular and Molecular Medicine,Boston Children's Hospital

Journal (Biochemistry)

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Daniel Bruce¹, Emily Cardew¹, Stefanie Freitag-Pohl², Ehmke Pohl^1,2

¹Department of Biosciences,Durham University, ²Department of Chemistry,Durham University

Journal (Biochemistry)

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Christophe Jung¹, Max Schnepf¹, Peter Bandilla¹, Ulrich Unnerstall¹, Ulrike Gaul¹

¹Gene Center and Department of Biochemistry, Center for Protein Science Munich (CIPSM),Ludwig-Maximilians-Universität München

Journal (Biochemistry)

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Patrick Rosendahl Andreassen¹, Jens Sivkær Pettersen¹, Mikkel Jørgensen¹

¹Department of Biochemistry and Molecular Biology,University of Southern Denmark

Journal (Biochemistry)

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Micah P. Stumme-Diers¹, Tommy Stormberg¹, Zhiqiang Sun¹, Yuri L. Lyubchenko¹

¹Department of Pharmaceutical Sciences,University of Nebraska Medical Center

Journal (Biochemistry)

Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

K. Martin Chow¹, Sidney W. Whiteheart¹, Jeffrey R. Smiley², Savita Sharma¹, Kathy Boaz², Meggie J. Coleman², Alvina Maynard³, Louis B. Hersh¹, Craig W. Vander Kooi¹

¹Department of Molecular and Cellular Biochemistry,University of Kentucky, ²Division of Laboratory Animal Resources,University of Kentucky, ³River Hill Ranch

Journal (Biochemistry)

Isolation of F₁-ATPase from the Parasitic Protist Trypanosoma brucei

Ondřej Gahura¹, Alena Zíková^1,2

¹Biology Centre,Czech Academy of Science, Institute of Parasitology, ²Faculty of Science,University of South Bohemia

Journal (Biochemistry)

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

William D. McCaig¹, Matthew A. Deragon¹, Robert J. Haluska Jr,¹, Alexa L. Hodges¹, Payal S. Patel¹, Timothy J. LaRocca¹

¹Department of Basic and Clinical Sciences,Albany College of Pharmacy and Health Sciences

Journal (Biochemistry)

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Eva Königshausen¹, Sebastian A. Potthoff¹, Raphael Haase¹, Catherine Meyer-Schwesinger², Ernest Kaufmann¹, L. Christian Rump¹, Johannes Stegbauer¹, Lorenz Sellin¹, Ivo Quack¹, Magdalena Woznowski¹

¹Department of Nephrology, Medical Faculty,Heinrich-Heine-University, ²Institute of Cellular and Integrative Physiology,University Medical Center Hamburg-Eppendorf

Journal (Biochemistry)

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Beáta Bozóki*^1,2, János András Mótyán*¹, Márió Miczi¹, Lívia Diána Gazda¹, József Tőzsér¹

¹Department of Biochemistry and Molecular Biology, Faculty of Medicine,University of Debrecen, ²Biotechnology Research Department,Gedeon Richter Plc

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