Imaging the Motion of Fully Reconstituted Cdc45/Mcm2-7/GINS (CMG)

July 26, 2024

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

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1Department of Bionanoscience, Kavli Institute of Nanoscience,Delft University of Technology, 2Chromosome Replication Laboratory,Francis Crick Institute, 3Department of Physics and Kavli Institute of Nanoscience Discovery,University of Oxford

Summary

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We report a hybrid ensemble and single-molecule assay to directly image and quantify the motion of fluorescently labeled, fully reconstituted Cdc45/Mcm2-7/GINS (CMG) helicase on linear DNA molecules held in place in an optical trap.

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