JOVE JOURNAL

Developmental Biology

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Journal (Developmental Biology)

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Frederic Strobl^1,2,3, Selina Klees^1,2,3, Ernst H. K. Stelzer^1,2,3

¹Physical Biology,Buchmann Institute for Molecular Life Sciences (BMLS), ²Cluster of Excellence Frankfurt,Macromolecular Complexes, ³Goethe-Universität Frankfurt am Main – Campus Riedberg

Journal (Developmental Biology)

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Tim Pieters^1,2,3,4, Lieven Haenebalcke^1,2, Kenneth Bruneel^1,2,4, Niels Vandamme^1,2,4, Tino Hochepied^1,2, Jolanda van Hengel⁵, Dagmar Wirth⁶, Geert Berx^1,2,4, Jody J. Haigh⁷, Frans van Roy^1,2,4, Steven Goossens^1,2,3,4

¹Department of Biomedical Molecular Biology,Ghent University, ²Inflammation Research Center,VIB, ³Center for Medical Genetics,Ghent University Hospital, ⁴Cancer Research Institute Ghent (CRIG), ⁵Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences,Ghent University, ⁶Helmholtz Center for Infection Research, ⁷Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology,Monash University and Alfred Health Alfred Centre

Journal (Developmental Biology)

Dissection of Larval Zebrafish Gonadal Tissue

Xinjian Wang*¹, Sijie Chen*¹, Wei Zhang¹, Yiyuan Ren¹, Quan Zhang¹, Gang Peng¹

¹Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science,Fudan University

Journal (Developmental Biology)

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Junning Yang¹, Chuanshen Wu², Ioana Stefanescu¹, Arie Horowitz^1,3

¹Department of Medicine, Cardeza Vascular Research Center,Sidney Kimmel Medical College, Thomas Jefferson University, ²Department of Molecular Cardiology,Cleveland Clinic Foundation, ³Department of Cancer Biology, Cardeza Vascular Research Center,Sidney Kimmel Medical College, Thomas Jefferson University

Journal (Developmental Biology)

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Elaine L. Welch*¹, Celeste C. Eno*¹, Sreelaja Nair², Robin E. Lindeman³, Francisco Pelegri¹

¹Laboratory of Genetics,University of Wisconsin-Madison, ²Department of Biological Sciences,Tata Institute of Fundamental Research, ³Department of Genetics, Cell Biology, and Development,University of Minnesota

Journal (Developmental Biology)

Surgical Ablation Assay for Studying Eye Regeneration in Planarians

Jacob M. Morton*¹, Marwa A. Saad*¹, Wendy S. Beane¹

¹Department of Biological Sciences,Western Michigan University

Journal (Developmental Biology)

Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster

Eisuke Imura*¹, Yuto Yoshinari*¹, Yuko Shimada-Niwa*², Ryusuke Niwa³

¹Graduate School of Life and Environmental Sciences,University of Tsukuba, ²Life Science Center of Tsukuba Advanced Research Alliance,University of Tsukuba, ³Faculty of Life and Environmental Sciences,University of Tsukuba

Journal (Developmental Biology)

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

Elke Gabriel¹, Jay Gopalakrishnan^1,2

¹Center for Molecular Medicine Cologne,University of Cologne, ²Institute for Biochemistry I,Medical School of University of Cologne

Journal (Developmental Biology)

Using Primary Neurosphere Cultures to Study Primary Cilia

Issei S. Shimada¹, Hemant Badgandi¹, Bandarigoda N. Somatilaka¹, Saikat Mukhopadhyay¹

¹Department of Cell Biology,University of Texas Southwestern Medical Center

Journal (Developmental Biology)

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Naimisha Beeravolu^1,2, Christina McKee^1,2, Ali Alamri^1,2, Sasha Mikhael³, Christina Brown^1,2, Mick Perez-Cruet^2,4, G. Rasul Chaudhry^1,2

¹Department of Biological Sciences,Oakland University, ²OU-WB Institute for Stem Cell and Regenerative Medicine, ³Department of Obstetrics and Gynecology,St. John Provindence - Providence Park Hospital, ⁴Department of Neurosurgery,Beaumont Health System

Journal (Developmental Biology)

Live Confocal Imaging of Developing Arabidopsis Flowers

Nathanaël Prunet¹

¹Department of Biology and Biological Engineering,California Institute of Technology

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