JOVE JOURNAL

Developmental Biology

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Journal (Developmental Biology)

Isolation and Sectioning of Bone Tissue from Mouse for Transcriptomic Studies

Luigi Mancinelli^1,2, Karen Elizabeth Schoedel^3,4, Kurt Richard Weiss^5,6,7, Giuseppe Intini^1,2,8,9,10

¹Department of Periodontics and Preventive Dentistry,University of Pittsburgh School of Dental Medicine, ²Center for Craniofacial Regeneration,University of Pittsburgh School of Dental Medicine, ³UPMC Presbyterian, ⁴UPMC Shadyside, ⁵Musculoskeletal Oncology Laboratory, Department of Orthopaedic Surgery,University of Pittsburgh, ⁶UPMC Hillman Cancer Center, ⁷Departments of Anatomic Pathology and General Surgical Oncology,University of Pittsburgh, ⁸Department of Medicine,University of Pittsburgh School of Medicine, ⁹University of Pittsburgh UPMC Hillman Cancer Center, ¹⁰McGowan Institute for Regenerative Medicine,University of Pittsburgh

Journal (Developmental Biology)

Methods to Enable Spatial Transcriptomics of Bone Tissues

Luigi Mancinelli^1,2, Karen Elizabeth Schoedel^3,4, Kurt Richard Weiss^5,6,7, Giuseppe Intini^1,2,8,9,10

¹Department of Periodontics and Preventive Dentistry,University of Pittsburgh School of Dental Medicine, ²Center for Craniofacial Regeneration,University of Pittsburgh School of Dental Medicine, ³UPMC Presbyterian, ⁴UPMC Shadyside, ⁵Musculoskeletal Oncology Laboratory, Department of Orthopaedic Surgery,University of Pittsburgh, ⁶UPMC Hillman Cancer Center, ⁷Departments of Anatomic Pathology and General Surgical Oncology,University of Pittsburgh, ⁸Department of Medicine,University of Pittsburgh School of Medicine, ⁹University of Pittsburgh UPMC Hillman Cancer Center, ¹⁰McGowan Institute for Regenerative Medicine,University of Pittsburgh

Journal (Developmental Biology)

Generation of Germ-Free Zebrafish Models from Embryos

Pan-Pan Jia¹, Xiang-Ru Liu¹, Ming-Fei Wu¹, Yan Li¹, Lan-Chen Zhang¹, De-Sheng Pei¹

¹School of Public Health,Chongqing Medical University

Journal (Developmental Biology)

GF Artemia nauplii Preparation for Chronic GF Zebrafish Nutrition

Pan-Pan Jia¹, Xiang-Ru Liu¹, Ming-Fei Wu¹, Yan Li¹, Lan-Chen Zhang¹, De-Sheng Pei¹

¹School of Public Health,Chongqing Medical University

Journal (Developmental Biology)

Tracking Germ-Free Zebrafish Models Across All Life Stages

Pan-Pan Jia¹, Xiang-Ru Liu¹, Ming-Fei Wu¹, Yan Li¹, Lan-Chen Zhang¹, De-Sheng Pei¹

¹School of Public Health,Chongqing Medical University

Journal (Developmental Biology)

Generation, Maintenance, and Identification of Germ-Free Zebrafish Models from Larvae to Juvenile Stages

Pan-Pan Jia¹, Xiang-Ru Liu¹, Ming-Fei Wu¹, Yan Li¹, Lan-Chen Zhang¹, De-Sheng Pei¹

¹School of Public Health,Chongqing Medical University

Journal (Developmental Biology)

Mating and Feeding Rats for Maternal Exercise Training to Study Offspring Health

Ziqi Ni*¹, Jiaqi Cao*¹, Meiling Shan¹, Yanyan Zhang^1,2,3, Lijun Shi^1,2,3

¹Department of Exercise Physiology,Beijing Sport University, ²Laboratory of Sports Stress and Adaptation of General Administration of Sport,Beijing Sport University, ³Key Laboratory of Physical Fitness and Exercise, Ministry of Education,Beijing Sport University

Journal (Developmental Biology)

Prenatal Rat Swimming to Explore Offspring Health Benefits

Ziqi Ni*¹, Jiaqi Cao*¹, Meiling Shan¹, Yanyan Zhang^1,2,3, Lijun Shi^1,2,3

¹Department of Exercise Physiology,Beijing Sport University, ²Laboratory of Sports Stress and Adaptation of General Administration of Sport,Beijing Sport University, ³Key Laboratory of Physical Fitness and Exercise, Ministry of Education,Beijing Sport University

Journal (Developmental Biology)

Assessing the Physical Traits of Fetuses Obtained from Swim-Trained Pregnant Rats

Ziqi Ni*¹, Jiaqi Cao*¹, Meiling Shan¹, Yanyan Zhang^1,2,3, Lijun Shi^1,2,3

¹Department of Exercise Physiology,Beijing Sport University, ²Laboratory of Sports Stress and Adaptation of General Administration of Sport,Beijing Sport University, ³Key Laboratory of Physical Fitness and Exercise, Ministry of Education,Beijing Sport University

Journal (Developmental Biology)

Swimming Exercise Protocol and Care Methods for Pregnant Rats

Ziqi Ni*¹, Jiaqi Cao*¹, Meiling Shan¹, Yanyan Zhang^1,2,3, Lijun Shi^1,2,3

¹Department of Exercise Physiology,Beijing Sport University, ²Laboratory of Sports Stress and Adaptation of General Administration of Sport,Beijing Sport University, ³Key Laboratory of Physical Fitness and Exercise, Ministry of Education,Beijing Sport University

Journal (Developmental Biology)

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

Miriam Wandres*^1,2, Denise Aigner*^1,2, Nicolai Kastelic^1,3, Anastasiya Boltengagen¹, Agnieszka Rybak-Wolf^1,3, Nikolaus Rajewsky^1,2

¹Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC), ²Institut für Biologie,Humboldt-Universität zu Berlin, ³Organoid platform, Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC)

Journal (Developmental Biology)

Isolation of Single Cells from Human Brain Organoids for Single-Cell RNA Sequencing

Miriam Wandres*^1,2, Denise Aigner*^1,2, Nicolai Kastelic^1,3, Anastasiya Boltengagen¹, Agnieszka Rybak-Wolf^1,3, Nikolaus Rajewsky^1,2

¹Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC), ²Institut für Biologie,Humboldt-Universität zu Berlin, ³Organoid platform, Berlin Institute for Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine (MDC)

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