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Journal
/
Developmental Biology
/
Protocol to Prepare Demineralized FFPE Bone Tissue Samples with RNA Integrity
/
Isolation and Sectioning of Bone Tissue from Mouse for Transcriptomic Studies
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Isolation and Sectioning of Bone Tissue from Mouse for Transcriptomic Studies
Isolation and Sectioning of Bone Tissue from Mouse for Transcriptomic Studies
Protocol to Prepare Demineralized FFPE Bone Tissue Samples with RNA Integrity
DOI:
10.3791/201777-v
•
06:33 min
•
May 03, 2024
•
Luigi Mancinelli
2
,
Karen Elizabeth Schoedel
4
,
Kurt Richard Weiss
6,7
,
Giuseppe Intini
2,8,9,10
1
Department of Periodontics and Preventive Dentistry
,
University of Pittsburgh School of Dental Medicine
,
2
Center for Craniofacial Regeneration
,
University of Pittsburgh School of Dental Medicine
,
3
UPMC Presbyterian
,
4
UPMC Shadyside
,
5
Musculoskeletal Oncology Laboratory, Department of Orthopaedic Surgery
,
University of Pittsburgh
,
6
UPMC Hillman Cancer Center
,
7
Departments of Anatomic Pathology and General Surgical Oncology
,
University of Pittsburgh
,
8
Department of Medicine
,
University of Pittsburgh School of Medicine
,
9
University of Pittsburgh UPMC Hillman Cancer Center
,
10
McGowan Institute for Regenerative Medicine
,
University of Pittsburgh
Tags
Mouse
Transcriptomic Studies
EDTA Treatment
Dehydration
Clearing
Paraffin Embedding
Microtome Sectioning
RNase Decontamination
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