A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing

Lucas Stalder; Fabian Koechl; Kerstin Hahn; Marc Sultan; Megana K. Prasad

doi:10.3791/65611

Journal

Biology

Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

July 28, 2023

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

01:58 min

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2,244 Views

Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing

02:02 min

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287 Views

Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues

03:38 min

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159 Views

Lucas Stalder¹, Fabian Koechl¹, Kerstin Hahn¹, Marc Sultan¹, Megana K. Prasad¹

¹Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

Summary

The study describes a simple, quick, and partially automated protocol to isolate high-quality nuclei from frozen mammalian tissues for downstream single nuclei RNA sequencing.

Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

Author Spotlight: Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues

Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing

Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues

Summary

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Article