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Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction
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Genetics
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JoVE Journal Genetics
Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

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07:36 min

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August 27, 2020

DOI:

10.3791/61383-v

DOI:
10.3791/61383-v

•

07:36 min
August 27, 2020

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1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, 3Department of Medical Physiology,Meiji Pharmaceutical University

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MicroRNAQuantitative Real-time PCRUnilateral Ureteral ObstructionRenal FibrosisSham SurgeryUreteral LigationKidney SampleHomogenizationPhenyl/guanidine-based Lysis Reagent

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Yanai, K., Kaneko, S., Ishii, H., Aomatsu, A., Ito, K., Hirai, K., Ookawara, S., Ishibashi, K., Morishita, Y. Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction. J. Vis. Exp. (162), e61383, doi:10.3791/61383 (2020).

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