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Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction
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07:36 min
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August 27, 2020
DOI:
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, 3Department of Medical Physiology,Meiji Pharmaceutical University
Summary
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We describe a method for evaluating the microRNA expression in the kidneys of mice with unilateral ureteral obstruction (UUO) by quantitative reverse-transcription polymerase chain reaction. This protocol is suitable for studying kidney microRNA expression profiles in mice with UUO and in the context of other pathological conditions.
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Yanai, K., Kaneko, S., Ishii, H., Aomatsu, A., Ito, K., Hirai, K., Ookawara, S., Ishibashi, K., Morishita, Y. Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction. J. Vis. Exp. (162), e61383, doi:10.3791/61383 (2020).
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