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Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis

Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis

English

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6,625 Views

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09:16 min

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June 18, 2020

DOI:

10.3791/61180-v

DOI:
10.3791/61180-v

•

09:16 min
June 18, 2020

•

15 Views
, , ,
1Department of Medicine,Monash University, 2Monash Micro Imaging,Monash University, 3Immunology Department,Monash Health

Summary

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Extracellular DNA (ecDNA) released during cell death is proinflammatory and contributes to inflammation. Measurement of ecDNA at the site of injury can determine the efficacy of therapeutic treatment in the target organ. This protocol describes the use of a machine learning tool to automate measurement of ecDNA in kidney tissue.

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Cite this Article

O'Sullivan, K. M., Creed, S., Gan, P., Holdsworth, S. R. Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis. J. Vis. Exp. (160), e61180, doi:10.3791/61180 (2020).

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