/
/
One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy
This content is Free Access.
10,936 Views
•
06:47 min
•
October 23, 2017
DOI:
Summary
Automatically generated
Research on ionizing radiation (IR)-induced clonogenic cell death is important for understanding the effects of IR on malignant tumors and normal tissues. Here, we describe a one-step assay for assessing the major modes of IR-induced clonogenic cell death based on morphology of 4',6-diamidino-2-phenylindole dihydrochloride (DAPI)-stained nuclei visualized by fluorescence microscopy.
Read Article
Cite this Article
Kobayashi, D., Shibata, A., Oike, T., Nakano, T. One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy. J. Vis. Exp. (128), e56338, doi:10.3791/56338 (2017).
Copy