Journal
/
/
Immunoblot Analysis
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Immunoblot Analysis

Immunoblot Analysis

67,830 Views

16:01 min

June 20, 2008

DOI:

16:01 min
June 20, 2008

55 Views
,

Summary

Automatically generated

Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. This video provides protocols for protein separation, blotting proteins onto membranes, immunoprobing, and visualization using chromogenic or chemiluminescent substrates.

Read Article