This content is Free Access.
67,930 Views
•
16:01 min
•
June 20, 2008
DOI:
Summary
Automatically generated
Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. This video provides protocols for protein separation, blotting proteins onto membranes, immunoprobing, and visualization using chromogenic or chemiluminescent substrates.
Explore More Videos
Immunoblot AnalysisWestern BlottingProteinsAntigen-antibody RecognitionSolubilizationElectrophoretic SeparationGel ElectrophoresisNitrocellulosePVDFNylonSpecific AntigensPolyclonal AntibodiesMonoclonal AntibodiesSensitivityProtein SeparationMembrane BlottingImmunoprobingVisualizationChromogenic SubstratesChemiluminescent Substrates
Read Article
Cite this Article
Gallagher, S., Chakavarti, D. Immunoblot Analysis. J. Vis. Exp. (16), e759, doi:10.3791/759 (2008).
Copy