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Assemblaggio modulare basato su CRISPR per la costruzione ad alto rendimento di una libreria di plasmidi UAS-cDNA/ORF
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01:23 min
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May 17, 2024
DOI:
1Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 2Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 3Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School,University of South China, 4Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
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Xu, S., Chen, M., Chen, G., Luo, S., Xue, W., Liu, X., Wang, J., Wei, P. CRISPR-Based Modular Assembly for High-Throughput Construction of a UAS-cDNA/ORF Plasmid Library. J. Vis. Exp. (207), e66581, doi:10.3791/66581 (2024).
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