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Journal
/
Biochemistry
/
FRET Analysis of IDR Structural Sensitivity to Study Hyperosmotic Stress in Yeast
/
Author Spotlight: Unlocking the World of Intrinsically Disordered Regions with Cellular Sensing and Responses
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET
Author Spotlight: Unlocking the World of Intrinsically Disordered Regions with Cellular Sensing and Responses
FRET Analysis of IDR Structural Sensitivity to Study Hyperosmotic Stress in Yeast
DOI:
10.3791/66275-v
•
01:49 min
•
January 12, 2024
•
Constanza Enriquez-Toledo*
1
,
Cesar A. Ponce-Diego*
1
,
Cesar L. Cuevas-Velazquez
1
Departamento de Bioquímica, Facultad de Química
,
Universidad Nacional Autónoma de México
Tags
Intrinsically Disordered Regions
Structural Sensitivity
Hyperosmotic Stress
FRET
Conformational Ensemble
Biophysical Techniques
Cell Biology
Saccharomyces Cerevisiae
Osmolyte
Fluorescence Resonance Energy Transfer
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Author Spotlight: Unlocking the World of Intrinsically Disordered Regions with Cellular Sensing and Responses
Yeast Cell Preparation for FRET Assay for Hyperosmotic Stress Analysis
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