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FRET Analysis of IDR Structural Sensitivity to Study Hyperosmotic Stress in Yeast
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Author Spotlight: Unlocking the World of Intrinsically Disordered Regions with Cellular Sensing and Responses
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Biochimie
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Journal JoVE Biochimie
Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET

Author Spotlight: Unlocking the World of Intrinsically Disordered Regions with Cellular Sensing and Responses

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01:49 min

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January 12, 2024

DOI:

10.3791/66275-v

DOI:
10.3791/66275-v

•

01:49 min
January 12, 2024

•

8 Views
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1Departamento de Bioquímica, Facultad de Química,Universidad Nacional Autónoma de México

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Intrinsically Disordered RegionsStructural SensitivityHyperosmotic StressFRETConformational EnsembleBiophysical TechniquesCell BiologySaccharomyces CerevisiaeOsmolyteFluorescence Resonance Energy Transfer

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Enriquez-Toledo, C., Ponce-Diego, C. A., Cuevas-Velazquez, C. L. Estimation of Structural Sensitivity of Intrinsically Disordered Regions in Response to Hyperosmotic Stress in Living Cells Using FRET. J. Vis. Exp. (203), e66275, doi:10.3791/66275 (2024).

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