/

/

/

Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach

JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry

Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation

Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach

3,763 Views

•

01:15 min

•

December 29, 2023

DOI:

10.3791/66197-v

DOI:

10.3791/66197-v

•

01:15 min

December 29, 2023

•

37 Views

Trinity Cookis¹, Paul Sauer^2,3, Simon Poepsel^4,5, Bong-Gyoon Han⁶, Dominik A. Herbst^1,2,6, Robert Glaeser⁶, Eva Nogales^1,2,3,6

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²California Institute for Quantitative Biology (QB3),University of California, Berkeley, ³Howard Hughes Medical Institute,University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital,University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD),University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division,Lawrence Berkeley National Laboratory

Explore More Videos

Cryo-electron Microscopy Sample Preparation Streptavidin-affinity Grid Biotinylation Two-dimensional Streptavidin Crystal Air-water Interface Sample Denaturation Preferred Orientation Sample Concentration Protein Complex Purification

Read Article