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Journal
/
Biochemie
/
Fabrication of Streptavidin Affinity Grids for Cryo-EM Structural Studies
/
Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach
JoVE Journal
Biochemie
This content is Free Access.
JoVE Journal
Biochemie
Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation
Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach
Fabrication of Streptavidin Affinity Grids for Cryo-EM Structural Studies
DOI:
10.3791/66197-v
•
01:15 min
•
December 29, 2023
•
Trinity Cookis
,
Paul Sauer
3
,
Simon Poepsel
5
,
Bong-Gyoon Han
,
Dominik A. Herbst
2,6
,
Robert Glaeser
,
Eva Nogales
2,3,6
1
Department of Molecular and Cell Biology
,
University of California, Berkeley
,
2
California Institute for Quantitative Biology (QB3)
,
University of California, Berkeley
,
3
Howard Hughes Medical Institute
,
University of California, Berkeley
,
4
Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital
,
University of Cologne
,
5
Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD)
,
University of Cologne
,
6
Molecular Biophysics and Integrative Bio-Imaging Division
,
Lawrence Berkeley National Laboratory
Tags
Cryo-electron Microscopy
Sample Preparation
Streptavidin-affinity Grid
Biotinylation
Two-dimensional Streptavidin Crystal
Air-water Interface
Sample Denaturation
Preferred Orientation
Sample Concentration
Protein Complex Purification
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Author Spotlight: Enhancing Cryo-EM Sample Preparation with Streptavidin-Biotin Approach
Designing Streptavidin Affinity Grids for Cryo-Electron Microscopy
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