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Yüksek Çözünürlüklü Yapı Belirleme için Tek Katmanlı Grafenin Kriyo-Elektron Mikroskobu Izgaralarına Uygulanması

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Application of Monolayer Graphene to Cryo-Electron Microscopy Grids for High-resolution Structure Determination

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Yüksek Çözünürlüklü Yapı Belirleme için Tek Katmanlı Grafenin Kriyo-Elektron Mikroskobu Izgaralarına Uygulanması

732 Views

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01:38 min

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November 10, 2023

DOI:

10.3791/66023-v

DOI:

10.3791/66023-v

•

01:38 min

November 10, 2023

•

9 Views

Andrew V. Grassetti*¹, Mira B. May*¹, Joseph H. Davis²

¹Department of Biology,Massachusetts Institute of Technology, ²Program in Computational and Systems Biology,Massachusetts Institute of Technology

Tags

Cryo-electron Microscopy Graphene Monolayer Sample Preparation Particle Localization Air-water Interface Grid Surface Sample Concentration Background Noise Affinity Handles Cell Lysates Cryogenic Electron Microscopy Holey Carbon Foil Vitreous Ice Image Processing Near-atomic Resolution Sample Preparation Bottleneck Graphene-coated Grids

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