Journal
/
生物化学
/
Coating CryoEM Grids with Monolayer Graphene to Improve Cryo-Sample Preparation
/
Author Spotlight: Enhancing CryoEM Sample Preparation Using Graphene Monolayer on Microscopy Grids
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Application of Monolayer Graphene to Cryo-Electron Microscopy Grids for High-resolution Structure Determination

Author Spotlight: Enhancing CryoEM Sample Preparation Using Graphene Monolayer on Microscopy Grids

821 Views

•

01:38 min

•

November 10, 2023

DOI:

10.3791/66023-v

DOI:
10.3791/66023-v

•

01:38 min
November 10, 2023

•

24 Views
, ,
1Department of Biology,Massachusetts Institute of Technology, 2Program in Computational and Systems Biology,Massachusetts Institute of Technology

Explore More Videos

Cryo-electron MicroscopyGrapheneMonolayerSample PreparationParticle LocalizationAir-water InterfaceGrid SurfaceSample ConcentrationBackground NoiseAffinity HandlesCell LysatesCryogenic Electron MicroscopyHoley Carbon FoilVitreous IceImage ProcessingNear-atomic ResolutionSample Preparation BottleneckGraphene-coated Grids

Read Article

Cite this Article

Grassetti, A. V., May, M. B., Davis, J. H. Application of Monolayer Graphene to Cryo-Electron Microscopy Grids for High-resolution Structure Determination. J. Vis. Exp. (201), e66023, doi:10.3791/66023 (2023).

Download .ris file

Copy

Share Video

.

Copy