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Isolating Small Extracellular Vesicles and Extracting Their Peptidome

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Identificación de péptidos de pequeñas vesículas extracelulares a partir de macrófagos derivados de la médula ósea

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived Macrophages

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Identificación de péptidos de pequeñas vesículas extracelulares a partir de macrófagos derivados de la médula ósea

Isolating Small Extracellular Vesicles and Extracting Their Peptidome

DOI:

10.3791/65521-v

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01:19 min

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June 30, 2023

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Jiale Cheng², Jiong Zhu², Yujiao Liu³, Chen Yang³, Yuehui Zhang, Yuchen Liu, Chaozhi Jin, Jian Wang²

¹School of Basic Medical Sciences,Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing),Beijing Institute of Lifeomics, ³School of Life Sciences,Hebei University

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Keywords: Small Extracellular Vesicles SEVs Peptidome Bone Marrow-derived Macrophages Innate Immunity Differential Ultracentrifugation LC-MS/MS Intercellular Communication Antimicrobial Components Peptides

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