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Journal
/
Biochemistry
/
Isolating Small Extracellular Vesicles and Extracting Their Peptidome
/
Isolating Small Extracellular Vesicles (SEVs) by Differential Ultracentrifugation
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Isolating Small Extracellular Vesicles (sEVs) by Differential Ultracentrifugation
Isolating Small Extracellular Vesicles (SEVs) by Differential Ultracentrifugation
Isolating Small Extracellular Vesicles and Extracting Their Peptidome
DOI:
10.3791/200347-v
•
04:25 min
•
June 30, 2023
•
Jiale Cheng
2
,
Jiong Zhu
2
,
Yujiao Liu
3
,
Chen Yang
3
,
Yuehui Zhang
,
Yuchen Liu
,
Chaozhi Jin
,
Jian Wang
2
1
School of Basic Medical Sciences
,
Anhui Medical University
,
2
State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing)
,
Beijing Institute of Lifeomics
,
3
School of Life Sciences
,
Hebei University
Tags
Extracellular Vesicles
SEVs
Differential Ultracentrifugation
Macrophages
FBS
DMEM
PBS
Centrifugation
Ultracentrifugation
Pellets
Supernatant
Debris
Microvesicles
Protein Preparation
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