Journal
/
Genetics
/
A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells
/
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

A Comprehensive Approach to Studying the Promoter Interactome in Scarce Cells

DOI:
10.3791/65316-v

•

01:54 min

•

April 21, 2023

•

, , , ,
1Josep Carreras Leukaemia Research Institute, 2Barcelona Supercomputing Center, 3Catalan Institution for Research and Advanced Studies (ICREA), 4Institute for Health Science Research Germans Trias i Pujol

Tags

LiCHi-CChromatin InteractionsGenome OrganizationGene RegulationRegulatory ElementsPromoter-centricSpatiotemporalRare Cell PopulationsLow InputCapture Hi-C

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Lysis and Digestion of Scarce Cells

Ligation and Decrosslinking of Scarce Cell DNA

Sonication and Biotin Pull-Down of Scarce Cells DNA

DATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA

Library Capture, Biotin Pull-Down, and PCR Amplification Using Scarce Cell DNA Library

Read Article