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包封狂犬病抗原的脉冲聚合物微粒的制备
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies Antigen
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包封狂犬病抗原的脉冲聚合物微粒的制备

English

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827 Views

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07:44 min

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May 12, 2023

DOI:

10.3791/65147-v

DOI:
10.3791/65147-v

•

07:44 min
May 12, 2023

•

6 Views
, , , , , , ,
1Department of Bioengineering,Rice University, 2Particles for Humanity, 3IMPACT Technology Development, 4Department of Chemistry,Rice University

Summary

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该方法描述了将狂犬病抗原封装到具有结构和材料特性的可生物降解聚合物微粒中，这些微粒能够在预定延迟后实现脉动释放。对从颗粒核心回收的抗原进行酶联免疫吸附测定 （ELISA） 评估，通过颗粒制造证实存在完整的三聚体狂犬病病毒糖蛋白。

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Cite this Article

Graf, T. P., Kadasia, K., Melhorn, S., Kessler, E., Yang, H., Baryakova, T., Brady, S., McHugh, K. J. Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies Antigen. J. Vis. Exp. (195), e65147, doi:10.3791/65147 (2023).

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