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Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies Antigen
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JoVE Journal Bioingegneria
Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies Antigen

Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies Antigen

865 Views

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07:44 min

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May 12, 2023

DOI:

10.3791/65147-v

DOI:
10.3791/65147-v

•

07:44 min
May 12, 2023

•

6 Views
, , , , , , ,
1Department of Bioengineering,Rice University, 2Particles for Humanity, 3IMPACT Technology Development, 4Department of Chemistry,Rice University

Summary

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This method describes the encapsulation of the rabies antigen into biodegradable polymeric microparticles with structural and material properties that enable pulsatile release after a predetermined delay. Enzyme-linked immunosorbent assay (ELISA) assessment of the antigen retrieved from the particle core confirms the presence of intact trimeric rabies virus glycoprotein through particle fabrication.

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Cite this Article

Graf, T. P., Kadasia, K., Melhorn, S., Kessler, E., Yang, H., Baryakova, T., Brady, S., McHugh, K. J. Fabrication of Pulsatile Polymeric Microparticles Encapsulating Rabies Antigen. J. Vis. Exp. (195), e65147, doi:10.3791/65147 (2023).

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