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使用基于光学的系统对水凝胶嵌入的完整小鼠肌纤维进行高通量收缩测量
JoVE Journal
Biology
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JoVE Journal Biology
High-Throughput Contractile Measurements of Hydrogel-Embedded Intact Mouse Muscle Fibers Using an Optics-Based System
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使用基于光学的系统对水凝胶嵌入的完整小鼠肌纤维进行高通量收缩测量

English

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1,095 Views

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07:35 min

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May 05, 2023

DOI:

10.3791/65103-v

DOI:
10.3791/65103-v

•

07:35 min
May 05, 2023

•

10 Views
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1Department of Physiology,Amsterdam UMC, 2Amsterdam Cardiovascular Sciences, Heart Failures and Arrhythmias,Amsterdam UMC, 3Amsterdam Movement Sciences, Tissue Function and Regeneration,Amsterdam UMC, 4Discipline of Child and Adolescent Health, Faculty of Health and Medicine,University of Sydney

Summary

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骨骼肌功能可以通过量化孤立的肌肉纤维的收缩力来评估，传统上使用费力的低通量方法。在这里，我们描述了一种基于光学的高通量方法来量化水凝胶嵌入的肌肉纤维的收缩性。这种方法在药物筛选和治疗开发方面有应用。

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Vonk, L. A., Esen, O., Yuen, M., Kirby, T. J. High-Throughput Contractile Measurements of Hydrogel-Embedded Intact Mouse Muscle Fibers Using an Optics-Based System. J. Vis. Exp. (195), e65103, doi:10.3791/65103 (2023).

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