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High-Throughput Contractile Measurements of Hydrogel-Embedded Intact Mouse Muscle Fibers Using an Optics-Based System
 

High-Throughput Contractile Measurements of Hydrogel-Embedded Intact Mouse Muscle Fibers Using an Optics-Based System

Article DOI: 10.3791/65103-v • 07:35 min • May 5th, 2023
May 5th, 2023 •
*1, *1, 1,4, 1,2,3
1Department of Physiology, Amsterdam UMC, 2Amsterdam Cardiovascular Sciences, Heart Failures and Arrhythmias, Amsterdam UMC, 3Amsterdam Movement Sciences, Tissue Function and Regeneration, Amsterdam UMC, 4Discipline of Child and Adolescent Health, Faculty of Health and Medicine, University of Sydney
* These authors contributed equally

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Skeletal muscle function can be assessed by quantifying the contractility of isolated muscle fibers, traditionally using laborious, low-throughput approaches. Here, we describe an optics-based, high-throughput method to quantify the contractility of hydrogel-embedded muscle fibers. This approach has applications for drug screening and therapeutic development.

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High-throughput Contractile Measurements Hydrogel-embedded Intact Mouse Muscle Fibers Optics-based System Genetic Mutations Muscle Fiber Function Drug Screening Studies Muscle Health Improvement Isolate And Measure Contractility Large Number Of Muscle Fibers Short Period Of Time Advanced Training Hydrogel Composition Environmental Cues Healthy And Diseased Muscle Initial Dissection Decrease In Viability Leander Vonk Research Technician Osman Esen PhD Candidate Laboratory Cutting The Lower Hind Leg Incision On The Skin Peel The Skin Towards The Toes Dissected Foot Seal Guard Dish Pre-warmed Dissection Medium Pinning The Foot Through The Skin Excise The Connective Tissue
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