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A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration
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Genetics
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JoVE Journal Genetics
A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

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1,071 Views

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09:24 min

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March 10, 2023

DOI:

10.3791/64927-v

DOI:
10.3791/64927-v

•

09:24 min
March 10, 2023

•

12 Views
, , , , ,
1Department of Medical Genetics,University of Wisconsin-Madison, 2McPherson Eye Research Institute,University of Wisconsin-Madison, 3Department of Ophthalmology,Duke University, 4Department of Cell Biology,Duke University

Summary

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Mouse models can be useful tools for investigating the biology of the retinal pigmented epithelium (RPE). It has been established that mice can develop an array of RPE pathologies. Here, we describe a phenotyping protocol to elucidate and quantify RPE pathologies in mice using light, transmission electron, and confocal microscopy.

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Retinal Pigmented EpitheliumAge-related Macular DegenerationMouse ModelLight Transmission Electron MicroscopyConfocal MicroscopyCardiac PerfusionEye Nucleation

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Landowski, M., Grindel, S., Hao, Y., Ikeda, S., Bowes Rickman, C., Ikeda, A. A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration. J. Vis. Exp. (193), e64927, doi:10.3791/64927 (2023).

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