Journal
/
Biochemistry
/
使用短生物素化RNA对RNA结合蛋白进行优化的定量下拉分析
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
Please note that all translations are automatically generated. Click here for the English version.

使用短生物素化RNA对RNA结合蛋白进行优化的定量下拉分析

English

Automatically Generated

2,073 Views

•

07:55 min

•

February 17, 2023

DOI:

10.3791/64923-v

DOI:
10.3791/64923-v

•

07:55 min
February 17, 2023

•

40 Views
, , , ,
1Center for Human Technology,Italian Institute of Technology (IIT), 2Department of Experimental Oncology,European Institute of Oncology (IEO), 3Department of Biology and Biotechnologies ‘Charles Darwin’,Sapienza University of Rome

Summary

Automatically generated

在这里，我们提出了一种优化的 体外 方法，使用来自人类细胞的总蛋白质提取物、涂有生物素化RNA的链霉亲和素珠和质谱分析来发现，定量和验证特定RNA序列的蛋白质相互作用物。

Explore More Videos

RNA-binding ProteinsPull-down AnalysisBiotinylated RNAProtein ComplexesHEK 293T CellsLysis BufferMagnetic BeadsYeast TRNARNA Oligonucleotide

Read Article

Cite this Article

Crua Asensio, N., Rizzieri, S., Cuomo, A., Tartaglia, G. G., Zacco, E. An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA. J. Vis. Exp. (192), e64923, doi:10.3791/64923 (2023).

Download .ris file

Copy

Share Video

.

Copy