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Neuroscience
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小鼠脉络丛的显微切割和全安装扫描电子显微镜可视化
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Microdissection and Whole Mount Scanning Electron Microscopy Visualization of Mouse Choroid Plexus
Please note that all translations are automatically generated.
Click here for the English version.
小鼠脉络丛的显微切割和全安装扫描电子显微镜可视化
DOI:
10.3791/64733-v
•
06:45 min
•
December 16, 2022
•
Elien Van Wonterghem*
1,2
,
Lien Van Hoecke*
1,2
,
Griet Van Imschoot
2
,
Daan Verhaege
2
,
Marlies Burgelman
2
,
Roosmarijn E. Vandenbroucke
2
1
VIB Center for Inflammation Research
,
VIB
,
2
Department of Biomedical Molecular Biology
,
Ghent University
Chapters
00:05
Introduction
00:59
Microdissection of the Choroid Plexus Out of the Lateral and Fourth Ventricle
03:47
Morphological Analysis of Choroid Plexus Tissue Using Scanning Electron Microscopy (SEM)
04:48
Results: Isolation of Choroid Plexus and Analysis of Cellular Structure
05:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
脉络丛(CP)是神经科学中研究不足的组织,在中枢神经系统的健康和疾病中起着关键作用。该协议描述了用于分离CP的显微切割技术,并使用扫描电子显微镜来获得其细胞结构的整体视图。
Tags
Microdissection
Whole Mount Scanning Electron Microscopy
Choroid Plexus
Brain Ventricles
Staining
Transcardial Perfusion
Mouse Brain
Cerebellum
Fourth Ventricle
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