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Real-Time Void Spot Assay
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Behavior
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JoVE Journal Behavior
Real-Time Void Spot Assay

Real-Time Void Spot Assay

DOI:
10.3791/64621-v

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06:39 min

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February 10, 2023

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, , , ,
1Renal-Electrolyte Division, Department of Medicine,University of Pittsburgh, 2Department of Cell Biology,University of Pittsburgh

Chapters

  • 00:05Introduction
  • 00:33Real-Time Void Spot Assay (RT-VSA) Recordings During the Light and Dark Phases of the Day
  • 02:57Analysis of the Experimental Mice Recordings
  • 04:53Results: Voiding Behavior of Mice
  • 05:56Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a new method to study mouse voiding behavior by incorporating video monitoring in the conventional void spot assay. This approach provides temporal, spatial, and volumetric information on the voiding events and details of mouse behavior during the light and dark phases of the day.

Tags

Real-time Void Spot AssayRT-VSAMouse Volume BehaviorVoiding EventsFilter PaperPlastic IglooMicrocentrifuge TubeDry ChowWater Gel PackVideo RecordingUltraviolet LightsVideo Surveillance SoftwareLight And Dark Phases

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