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使用高分辨率呼吸测定法实时分析原代人视网膜色素上皮细胞中的生物能量学
JoVE Journal
Biology
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JoVE Journal Biology
Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry
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使用高分辨率呼吸测定法实时分析原代人视网膜色素上皮细胞中的生物能量学

English

Automatically Generated

1,776 Views

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09:16 min

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February 03, 2023

DOI:

10.3791/64572-v

DOI:
10.3791/64572-v

•

09:16 min
February 03, 2023

•

7 Views
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1Schepens Eye Research Institute of Mass. Eye and Ear, 2Department of Ophthalmology,Harvard Medical School

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Real-time BioenergeticsPrimary Human Retinal Pigment Epithelial CellsHigh-resolution RespirometryOxphosGlycolysisAMDCell CultureRespirometry InstrumentMito Stress Test Assay

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Fitch, T. C., Frank, S. I., Li, Y. K., Saint-Geniez, M., Kim, L. A., Shu, D. Y. Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry. J. Vis. Exp. (192), e64572, doi:10.3791/64572 (2023).

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