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Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry
JoVE Journal
Biology
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JoVE Journal Biology
Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

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1,869 Views

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09:16 min

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February 03, 2023

DOI:

10.3791/64572-v

DOI:
10.3791/64572-v

•

09:16 min
February 03, 2023

•

11 Views
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1Schepens Eye Research Institute of Mass. Eye and Ear, 2Department of Ophthalmology,Harvard Medical School

Summary

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The metabolic status of human retinal pigment epithelial cells (H-RPE) reflects their health and function. Presented here is an optimized protocol for examining the real-time metabolic flux of H-RPE using high-resolution respirometry.

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Fitch, T. C., Frank, S. I., Li, Y. K., Saint-Geniez, M., Kim, L. A., Shu, D. Y. Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry. J. Vis. Exp. (192), e64572, doi:10.3791/64572 (2023).

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