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Analisi morfologica e composizionale di trappole extracellulari neutrofile indotte da stimoli microbici e chimici
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Immunology and Infection
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JoVE Journal Immunology and Infection
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
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Analisi morfologica e composizionale di trappole extracellulari neutrofile indotte da stimoli microbici e chimici

italiano

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2,088 Views

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14:05 min

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November 04, 2022

DOI:

10.3791/64522-v

DOI:
10.3791/64522-v

•

14:05 min
November 04, 2022

•

9 Views
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1Clinical Immunology Research Department of Biochemical Sciences Faculty,Universidad Autónoma “Benito Juárez” de Oaxaca, 2Department of Molecular Biomedicine,Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 3CONACYT- Medicine and Surgery Faculty,Universidad Autónoma “Benito Juárez” de Oaxaca, 4Molecular Immunology Research Department of Medicine and Surgery Faculty,Universidad Autónoma “Benito Juárez” de Oaxaca

Summary

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Presentato qui è un protocollo per l'induzione e l'analisi di trappole extracellulari (NET) di neutrofili in vitro . La quantificazione del DNA, della catelicidina (LL37) e dell'attività enzimatica ha prodotto dati che mostrano la variabilità nella composizione e morfologia dei NET indotta da stimoli microbici e chimici in condizioni controllate simili.

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Almaraz-Arreortua, A., Sosa-Luis, S. A., Ríos-Ríos, W. d. J., Romero-Tlalolini, M. d. l. Á., Aguilar-Ruiz, S. R., Baltiérrez-Hoyos, R., Torres Aguilar, H. Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli. J. Vis. Exp. (189), e64522, doi:10.3791/64522 (2022).

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