Journal
/
Cancer Research
/
Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

English

Automatically Generated

2,229 Views

•

10:13 min

•

December 02, 2022

DOI:

10.3791/64484-v

DOI:
10.3791/64484-v

•

10:13 min
December 02, 2022

•

6 Views
, , , , ,
1Department of Molecular Biology and Genetics,Aarhus University, 2Department of Clinical Medicine,Aarhus University, 3VPCIR Biosciences ApS, 4Department of Pathology,Aarhus University Hospital

Summary

Automatically generated

A protocol for the sensitive and quantitative detection of topoisomerase 1 activity using the rolling circle enhanced enzyme activity detection assay is described. The method allows detection of topoisomerase 1 activity from purified components or cell/tissue extracts. This protocol has wide-ranging applications in any field involving detection of enzymatic activity.

Explore More Videos

Rolling Circle AmplificationTopoisomerase 1 ActivityCrude Biological SamplesSimple And Fast DetectionAnti-cancerAnti-infectious DiseasesPrimer MixCircular SubstratesRecombinant Topoisomerase 1Cell ExtractSDSBuffer WashHybridization

Read Article

Cite this Article

Keller, J. G., Mizielinski, K., Petersen, K. V., Stougaard, M., Knudsen, B. R., Tesauro, C. Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples. J. Vis. Exp. (190), e64484, doi:10.3791/64484 (2022).

Download .ris file

Copy

Share Video

.

Copy