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Microamostragem em Análise de Proteínas de Baixa Abundância Baseada em Espectrometria de Massa
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

Microamostragem em Análise de Proteínas de Baixa Abundância Baseada em Espectrometria de Massa

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1,990 Views

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10:21 min

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January 13, 2023

DOI:

10.3791/64473-v

DOI:
10.3791/64473-v

•

10:21 min
January 13, 2023

•

2 Views
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1Department of Pharmacy,University of Oslo

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MicrosamplingTargeted Mass SpectrometryProtein AnalysisLow-abundance ProteinsAffinity CleanupProtein ProteolysisAntibodyPH AdjustmentMagnetic BeadsAntibody CouplingBorate BufferCoupling Buffer

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Ngo, H. B., Oulie, I., Reubsaet, L., Halvorsen, T. G. Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins. J. Vis. Exp. (191), e64473, doi:10.3791/64473 (2023).

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