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Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
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Developmental Biology
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JoVE Journal Developmental Biology
Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

English

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1,638 Views

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10:09 min

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September 13, 2022

DOI:

10.3791/64459-v

DOI:
10.3791/64459-v

•

10:09 min
September 13, 2022

•

1 Views
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1Department of Genetics,Rutgers, The State University of New Jersey, 2Human Genetics Institute of New Jersey, 3Department of Theriogenology, Faculty of Veterinary Medicine,Mansoura University

Summary

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Error in chromosome segregation is a common feature in oocytes. Therefore, studying the spindle assembly checkpoint gives important clues about the mechanisms needed to produce healthy eggs. The present protocol describes three complementary assays to evaluate spindle assembly checkpoint integrity in mouse oocytes.

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Cite this Article

Aboelenain, M., Schindler, K., Blengini, C. S. Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes. J. Vis. Exp. (187), e64459, doi:10.3791/64459 (2022).

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