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Multiparametrisk tumor organoid narkotika screening ved hjelp av Widefield Live-Cell Imaging for bulk og single-organoid analyse
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December 23, 2022
DOI:
1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON),University of Antwerp, 2Industrial Vision Lab,University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery,University Hospital Antwerp (UZA), 4Department of Oncology,University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT),University of Antwerp
Summary
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Denne protokollen beskriver en semi-automatisert metode for middels til høy gjennomstrømning organoid narkotika screenings og mikroskop-agnostisk, automatisert bildeanalyse programvare for å kvantifisere og visualisere multiparametriske, single-organoid narkotika responser for å fange intratumor heterogenitet.
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MultiparametricTumor OrganoidDrug ScreeningLive-cell ImagingBulk AnalysisSingle-organoid AnalysisStreamlined WorkflowAutomated AnalysisClinically RelevantPatient-derived Tumor OrganoidsPDTOExtracellular MatrixECMDissociationCell SuspensionPDAC OrganoidCulture MediumCell PlatingECM DomesY-27632Organoid Harvesting
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Cite this Article
Le Compte, M., Cardenas De La Hoz, E., Peeters, S., Smits, E., Lardon, F., Roeyen, G., Vanlanduit, S., Prenen, H., Peeters, M., Lin, A., Deben, C. Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis. J. Vis. Exp. (190), e64434, doi:10.3791/64434 (2022).
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