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Multiparametrisches Tumor-Organoid-Wirkstoff-Screening mit Weitfeld-Live-Cell-Imaging für die Bulk- und Einzelorganoid-Analyse
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1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON),University of Antwerp, 2Industrial Vision Lab,University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery,University Hospital Antwerp (UZA), 4Department of Oncology,University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT),University of Antwerp
Chapters
- 00:05Introduction
- 00:53Day 0: Preparation of the 2- or 3-Day-Old Organoids
- 02:55Days 2 – 3: Harvesting the 2- or 3-Day-Old Organoids
- 04:16Seeding the 2- or 3-Day-Old Organoids Using a Pipetting Robot
- 05:27Day 4: Drug Treatment and Reagent Dispensing with Digital Drug Dispenser
- 07:49Image Acquisition with the Live-Cell Imager and Analysis of Data
- 10:12Results: The Single-Organoid Dose-Response Analysis
- 12:03Conclusion
Dieses Protokoll beschreibt eine halbautomatische Methode für Organoid-Wirkstoff-Screenings mit mittlerem bis hohem Durchsatz und mikroskopunabhängige, automatisierte Bildanalysesoftware zur Quantifizierung und Visualisierung multiparametrischer, einzelorganoider Arzneimittelreaktionen zur Erfassung der intratumoralen Heterogenität.
Tags
Keywords:
MultiparametricTumor OrganoidDrug ScreeningLive-cell ImagingBulk AnalysisSingle-organoid AnalysisStreamlined WorkflowAutomated AnalysisClinically RelevantPatient-derived Tumor OrganoidsPDTOExtracellular MatrixECMDissociationCell SuspensionPDAC OrganoidCulture MediumCell PlatingECM DomesY-27632Organoid Harvesting
