/
/
Fluorescent In Situ Hybridization and 5-Ethynyl-2′-Deoxyuridine Labeling for Stem-Like Cells in the Hydrozoan Jellyfish Cladonema pacificum
A subscription to JoVE is required to view this content. Sign in or start your free trial.
2,877 Views
•
08:44 min
•
August 03, 2022
DOI:
Summary
Automatically generated
Here, we describe a protocol for visualizing stem-like proliferating cells in the jellyfish Cladonema. Whole-mount fluorescent in situ hybridization with a stem cell marker allows for the detection of stem-like cells, and 5-ethynyl-2'-deoxyuridine labeling enables the identification of proliferating cells. Together, actively proliferating stem-like cells can be detected.
Explore More Videos
Read Article
Cite this Article
Fujita, S., Kuranaga, E., Miura, M., Nakajima, Y. Fluorescent In Situ Hybridization and 5-Ethynyl-2'-Deoxyuridine Labeling for Stem-Like Cells in the Hydrozoan Jellyfish Cladonema pacificum. J. Vis. Exp. (186), e64285, doi:10.3791/64285 (2022).
Copy