Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

Bob A. Hersbach; Tatiana Simon; Giacomo Masserdotti

doi:10.3791/64175

Journal

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Neuroscience

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Isolement et reprogrammation neuronale directe des astrocytes de souris

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Neuroscience

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JoVE Journal Neuroscience

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

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Isolement et reprogrammation neuronale directe des astrocytes de souris

2,427 Views

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07:25 min

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July 07, 2022

DOI:

10.3791/64175-v

DOI:

10.3791/64175-v

•

07:25 min

July 07, 2022

•

6 Views

Bob A. Hersbach^1,2,3, Tatiana Simon², Giacomo Masserdotti^1,2

¹Institute of Stem Cell Research, Helmholtz Zentrum München,German Research Center for Environmental Health, ²Department of Physiological Genomics, Biomedical Center Munich,Ludwig-Maximilians University, ³Graduate School of Systemic Neurosciences, BioCenter,Ludwig-Maximilians University

Summary

Nous décrivons ici un protocole détaillé pour générer des cultures hautement enrichies d’astrocytes dérivés de différentes régions du système nerveux central de souris postnatales et leur conversion directe en neurones fonctionnels par l’expression forcée de facteurs de transcription.

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Isolement et reprogrammation neuronale directe des astrocytes de souris

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