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A Photodynamic Approach to Study Function of Intracellular Vesicle Rupture
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Photodynamic Approach to Study Function of Intracellular Vesicle Rupture

A Photodynamic Approach to Study Function of Intracellular Vesicle Rupture

English

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949 Views

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05:16 min

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March 17, 2023

DOI:

10.3791/63962-v

DOI:
10.3791/63962-v

•

05:16 min
March 17, 2023

•

12 Views
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1Inflammation Core Facility, Institute of Biomedical Sciences,Academia Sinica

Summary

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AlPcS2a-mediated chromophore-assisted laser inactivation (CALI) is a powerful tool for studying spatiotemporal damage of intracellular vesicles (IVs) in live cells.

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Photodynamic ApproachIntracellular Vesicle RuptureEndocytosisSignal PathwaysChromophore-assisted Laser InactivationSubcellular DamageHeLa CellsGal3-GFPAlPcS2aLysosomeConfocal Microscopy

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Cite this Article

Hung, Y., Wang, H., Wang, J., Hsu, C., Chen, H. A Photodynamic Approach to Study Function of Intracellular Vesicle Rupture. J. Vis. Exp. (193), e63962, doi:10.3791/63962 (2023).

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