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Isolamento di lisati proteici a cellule intere da processi facciali di topo e cellule mesenchimali palatali coltivate per l'analisi delle fosfoproteine
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
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Isolamento di lisati proteici a cellule intere da processi facciali di topo e cellule mesenchimali palatali coltivate per l'analisi delle fosfoproteine

italiano

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1,766 Views

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07:26 min

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April 01, 2022

DOI:

10.3791/63834-v

DOI:
10.3791/63834-v

•

07:26 min
April 01, 2022

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5 Views
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1Department of Craniofacial Biology, School of Dental Medicine,University of Colorado Anschutz Medical Campus

Summary

Automatically generated

Il protocollo presenta un metodo per isolare lisati proteici di cellule intere da processi facciali embrionali di topo sezionati o cellule mesenchimali palatali embrionali di topo in coltura ed eseguire il successivo western blotting per valutare i livelli di proteine fosforilate.

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Protein LysatesPhosphoprotein AnalysisFacial ProcessesPalatal Mesenchyme CellsCraniofacial DevelopmentMouse EmbryosProtein IsolationPhosphatase InhibitorsProtein PhosphorylationDissectionCell Culture

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Cite this Article

Rogers, M. A., Dennison, B. J. C., Fantauzzo, K. A. Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis. J. Vis. Exp. (182), e63834, doi:10.3791/63834 (2022).

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